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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

10.0K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

22.8K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Southern Blot02:57

Southern Blot

21.5K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
21.5K
Labeling DNA Probes03:31

Labeling DNA Probes

8.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Nov 15, 2025

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

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Advances in Proximity Ligation in situ Hybridization (PLISH).

Monica Nagendran1, Adam M Andruska1, Pehr B Harbury2

  • 1Department of Internal Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Stanford University School of Medicine, Stanford, California, USA.

Bio-Protocol
|March 4, 2021
PubMed
Summary

We updated Proximity Ligation in situ Hybridization (PLISH) technology to quantitatively measure single-cell gene expression in intact tissues. This allows researchers to map cell interactions and profiles within their native spatial context for better understanding of development and disease.

Keywords:
In situ hybridizationRNA localizationSingle cell analysisSpatial transcriptomics

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In Situ Hybridization for the Precise Localization of Transcripts in Plants
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Last Updated: Nov 15, 2025

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In Situ Hybridization for the Precise Localization of Transcripts in Plants
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Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
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Area of Science:

  • Molecular biology
  • Genomics
  • Tissue analysis

Background:

  • Understanding cellular function in tissues requires spatial molecular profiling.
  • Existing methods may lack sensitivity, scalability, or ease of use for in vivo studies.

Purpose of the Study:

  • To present an updated Proximity Ligation in situ Hybridization (PLISH) technology.
  • To enable quantitative, single-cell gene expression measurement within intact tissues.
  • To facilitate mapping of cell classes and their in vivo interactions.

Main Methods:

  • Proximity Ligation in situ Hybridization (PLISH) technology.
  • Quantitative measurement of single-cell gene expression.
  • Recording spatial information for each profiled cell.

Main Results:

  • The updated PLISH technology provides high sensitivity, specificity, and signal-to-noise ratio.
  • PLISH enables retrospective mapping of distinct cell classes within tissues.
  • Inference of in vivo cell-cell interactions is possible with PLISH.

Conclusions:

  • PLISH is a rapid, scalable, and user-friendly technology for spatial transcriptomics.
  • It empowers basic and clinical researchers to study cellular molecular profiles in their native context.
  • PLISH enhances understanding of tissue development, maintenance, and disease.