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Total Triglyceride Quantification in Caenorhabditis elegans.

Anjali Sandhu1, Varsha Singh1

  • 1Dept of Molecular Reproduction, Development, and Genetics, Indian Institute of Science, Bangalore 560012, India.

Bio-Protocol
|March 4, 2021
PubMed
Summary

This study presents a new method to measure total triglycerides in the nematode Caenorhabditis elegans. This assay aids in understanding lipid metabolism

Keywords:
C. elegansFatFluorometryMetabolismTriglycerides

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Area of Science:

  • Gerontology
  • Biochemistry
  • Molecular Biology

Background:

  • Lipid metabolism plays a key role in regulating longevity in Caenorhabditis elegans.
  • Understanding lipid profiles is crucial for aging research and associated pathologies.
  • A significant portion of lipid metabolism genes in C. elegans are conserved in humans.

Purpose of the Study:

  • To describe a novel protocol for quantifying total triglycerides in C. elegans.
  • To provide a sensitive method for assessing stored fat levels in whole worm lysates.
  • To enable investigations into how genetic and environmental factors influence lipid metabolism and fat storage.

Main Methods:

  • Development of a protocol to quantify picomoles of triglycerides.
  • Application of the assay to whole worm lysates of C. elegans.
  • Validation of the assay's sensitivity for detecting subtle changes in fat content.

Main Results:

  • The protocol successfully quantifies total triglycerides in C. elegans.
  • The assay is sensitive enough to detect changes not visible through microscopy.
  • This method allows for the study of genetic and environmental influences on stored fats.

Conclusions:

  • The developed triglyceride quantification assay is a valuable tool for C. elegans research.
  • This method can advance the understanding of lipid metabolism in aging and disease.
  • The protocol facilitates the study of factors affecting stored fat in a model organism with human gene homologs.