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The consensus rye microsatellite map with EST-SSRs transferred from wheat.

D O Vidakovic¹, D Perovic², T V Semilet³

¹Julius Kuehn-Institute (JKI), Quedlinburg, Germany University of Novi Sad, Department of Biology and Ecology, Novi Sad, Serbia.

Vavilovskii Zhurnal Genetiki I Selektsii

|March 4, 2021

Summary

This study integrated wheat EST-SSRs into rye genetic maps, successfully mapping 23 loci. A consensus rye map was constructed, enhancing genetic resources for rye and wheat breeding.

Keywords:

SSR Secale cereale Triticum aestivum genetic mapping microsatellite markers

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Last Updated: Nov 15, 2025

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Published on: April 20, 2018

Area of Science:

Plant genetics
Genomics
Molecular biology

Background:

Microsatellite (SSR) markers are crucial for gene mapping in rye, wheat-rye translocations, and triticale.
Existing rye chromosome maps have limited SSR loci, necessitating the integration of new markers.
Wheat EST-SSRs offer a potential source for novel SSR markers in rye.

Purpose of the Study:

To integrate wheat EST-SSRs (Expressed Sequence Tag-derived SSRs) into existing rye genetic maps.
To construct a comprehensive consensus rye microsatellite map.
To expand the available SSR markers for rye chromosome mapping.

Main Methods:

Utilized four rye mapping populations (P87/P105, N6/N2, N7/N2, N7/N6).
Employed CFE (EST-SSRs) primers for mapping.
Integrated mapping data to construct a consensus map.

Main Results:

Successfully mapped 23 Xcfe loci (wheat EST-SSRs) to specific rye chromosomes (1R-7R).
Identified orthologous positions for most mapped Xcfe loci relative to common wheat, considering evolutionary translocations.
Constructed a consensus rye microsatellite map comprising 123 SSRs, 12 SNPs, 118 RFLPs, and 2 isozyme loci.

Conclusions:

The integration of wheat EST-SSRs significantly enriches the rye genetic map.
The developed consensus map provides a valuable resource for gene mapping and QTL analysis in rye.
Orthologous SSR loci facilitate comparative genomics between wheat and rye.