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Related Experiment Video

Updated: Nov 15, 2025

A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function
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FLICK: an optimized plate reader-based assay to infer cell death kinetics.

Ryan Richards1, Megan E Honeywell1, Michael J Lee1

  • 1Program in Systems Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.

STAR Protocols
|March 4, 2021
PubMed
Summary

This study introduces a new method, FLICK, for accurately measuring cell death kinetics. FLICK uses a standard fluorescence plate reader, making it a high-throughput and accessible tool for drug sensitivity evaluation.

Keywords:
CancerCell-based assaysHigh-throughput screening

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Last Updated: Nov 15, 2025

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Area of Science:

  • Cell biology
  • Pharmacology
  • Biotechnology

Background:

  • Accurate drug sensitivity evaluation is crucial for effective treatment.
  • Current viability assays often measure metabolic activity, which doesn't directly reflect cell death.
  • Quantifying cell death kinetics offers a more precise measure of drug efficacy.

Purpose of the Study:

  • To develop a method for directly quantifying cell death kinetics.
  • To provide a high-throughput and accessible assay for drug sensitivity testing.
  • To overcome the limitations of traditional viability assays.

Main Methods:

  • Development of the fluorescence-based and lysis-dependent inference of cell death kinetics (FLICK) assay.
  • Utilizing a standard fluorescence plate reader for measurements.
  • Distinguishing between live and dead cells without complex microscopy or genetic labels.

Main Results:

  • FLICK accurately quantifies cell death kinetics.
  • The method retains the high-throughput nature of common viability assays.
  • FLICK provides broad accessibility due to its reliance on standard equipment.

Conclusions:

  • FLICK offers an improved approach to evaluating drug sensitivity by directly measuring cell death.
  • This method enhances the precision and accessibility of cell death kinetic analysis.
  • FLICK is a valuable tool for pharmacological research and drug development.