Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Concurrent positional dynamics and activity mapping of DNA-binding proteins.

Nature protocols·2026
Same author

Intermediate state formation of topologically associated chromatin domains using quantum annealing.

Scientific reports·2026
Same author

Photonic crystal band edge coupled enhanced fluorescence from magneto-plasmonic cryosoret nano-assemblies for ultra-sensitive detection.

APL materials·2026
Same author

Selective capture and digital counting of intact HIV pseudovirus using designer DNA nets, tethered motion, and photonic resonator interferometric scattering microscopy.

APL bioengineering·2026
Same author

Insights into Tardigrade Damage-Suppression Protein, Dsup.

Biomolecules·2026
Same author

Delineation of a novel assembly intermediate in Rous sarcoma virus integration pathway.

Nucleic acids research·2026
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Nov 15, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.5K

KERA: analysis tool for multi-process, multi-state single-molecule data.

Joseph Tibbs1, Mohamed Ghoneim2, Colleen C Caldwell3

  • 1Department of Physics, University of Northern Iowa, Cedar Falls, IA 50614, USA.

Nucleic Acids Research
|March 4, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed the Kinetic Event Resolving Algorithm (KERA) to analyze molecular interactions within cells. This software tool quantifies complex assembly dynamics, revealing crucial insights into macromolecular complex mechanisms and architecture.

More Related Videos

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.6K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.1K

Related Experiment Videos

Last Updated: Nov 15, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.5K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.6K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.1K

Area of Science:

  • Cellular and Molecular Biology
  • Biophysics
  • Biochemistry

Background:

  • Cellular molecular machines dynamically assemble and disassemble.
  • Single-molecule spectroscopy and fluorescence microscopy allow observation of molecular interactions.
  • Understanding the assembly and dynamics of macromolecular complexes is crucial.

Purpose of the Study:

  • To introduce the Kinetic Event Resolving Algorithm (KERA) software.
  • To provide a tool for organizing and analyzing single-molecule fluorescence trajectory data.
  • To enable detailed mechanistic insights into molecular complex assembly and dynamics.

Main Methods:

  • Development of the Kinetic Event Resolving Algorithm (KERA).
  • Utilizing single-molecule spectroscopy and total internal reflection fluorescence microscopy.
  • Analyzing discretized fluorescent trajectories from single-molecule experiments.

Main Results:

  • KERA organizes single-molecule data by transition patterns.
  • KERA displays dwell time data for interaction sequences.
  • KERA's utility demonstrated in analyzing DNA binding protein dynamics (RPA and XPD helicase).

Conclusions:

  • KERA facilitates the quantification of molecular interaction sequences.
  • The software aids in deducing spatial and temporal organization of molecular complexes.
  • KERA provides valuable information on the assembly, dynamics, and architecture of macromolecular complexes.