Schlegelella koreensis sp. nov., isolated from evaporator core of automobile air conditioning system

A white-coloured, aerobic, and rod-shaped bacterium, designated strain ID0723^T was isolated from evaporator core of automobile air conditioning system. The strain was Gram-stain-negative, catalase positive, oxidase negative, and grew at pH 5.5-9.5, at temperature 18-37 °C, and at 0-2.0% (w/v) NaCl concentration. The phylogenetic analysis and 16S rRNA gene sequence data revealed that the strain ID0723^T was affiliated to the genus Schlegelella, with the closest phylogenetic member being Schlegelella brevitalea DSM 7029 ^T (98.1% sequence similarity). The chemotaxonomic features of strain ID0723^T were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the main polar lipids; Q-8 as an only ubiquinone; and summed feature 3 (C_16:1ω7c and/or C_{16: 1}ω6c), C_16:0, and summed feature 8 (C_18:1ω7c/or C_18:1ω6c) as the major fatty acids. The average nucleotide identity (ANI) and in silico DNA-DNA hybridization values between strain ID0723^T and S. brevitalea DSM 7029 ^T were 74.8% and 20.0%, respectively, which were below the cut-off values of 95% and 70%, respectively. The DNA G + C content was 69.9 mol%. The polyphasic taxonomic data clearly indicated that strain ID0723^T represents a novel species in the genus Schlegelella for which the name Schlegelella koreensis sp. nov. is proposed, with the type strain ID0723^T (= KCTC 72731 ^T = NBRC 114611 ^T).