Cloning and Characterization of phzR Gene from Pseudomonas aeruginosa
Bingbing Pang1, Tingting Liu1, Wenjia Zhang1
1College of Life Science, Guangxi Normal University, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China.
Abstract:
A gene encoding phzR was isolated from a phenazine-producing bacterium Pseudomonas aeruginosa 2016NX1. This paper provided the full-length cDNA encoding phzR (GenBank Accession no., MW143078). The cDNA of the phzR contained an open reading frame (ORF) of 714 bp. The potential regulatory elements were predicted in the phzR promoter region. The deduced amino acid sequence of P. aeruginosa phzR showed significant homology to the known phzRs from different organisms. Gene overexpression analysis showed that the phenazine content was improved (44.39%) in comparison to wild-type 2016NX1.
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