Bioanalysis of plasma acetate levels without derivatization by LC-MS/MS

Area of Science:

• Biochemistry
• Analytical Chemistry
• Pharmacology

Background:

• Acetate ions play crucial roles in human physiology and have therapeutic potential.
• Accurate measurement of acetate is vital for understanding its biological significance.

Purpose of the Study:

• To develop and validate a rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying acetate ions in human plasma.
• To enable accurate acetate measurement without the need for chemical derivatization.

Main Methods:

• Human plasma samples were analyzed using LC-MS/MS.
• A C18 column and post-column infusion of methanolic ammonium hydroxide were employed.
• Acetate ions were detected using a low-resolution mass spectrometer in negative ion mode.

Main Results:

• The method demonstrated high accuracy and precision.
• A lower limit of quantitation of 9.7 μM was achieved.
• A linear dynamic range extending up to 339.6 μM was established.

Conclusions:

• The developed LC-MS/MS method provides a rapid and reliable approach for acetate quantification in human plasma.
• This method is suitable for metabolomic and pharmacometabolomic studies involving acetate and similar compounds.
• The method's adaptability allows for future analytical enhancements.