Silicon nanoparticles-based ratiometric fluorescence platform: Real-time visual sensing to ciprofloxacin and Cu²

In this work, a silicon nanoparticles (Si NPs)-based ratiometric fluorescence sensing platform was conveniently fabricated by simply mixing fluorescent Si NPs as co-ligands and reference signal with lanthanide metal ion Eu³⁺ as response signal. The introduction of ciprofloxacin (CIP) remarkably turned on the characteristic fluorescence of Eu³⁺ at 590 nm and 619 nm through the "antenna effect". At the same time, the blue emission of Si NPs at 445 nm kept comparatively stable. A good linear relationship between the ratio fluorescence intensity and CIP concentration in the range of 0.211-132.4 μM with a limit of detection (LOD) of 89 nM was obtained. In the presence of Cu²⁺, the fluorescence emission of Eu³⁺ was sharply turned off because of the stronger coordination ability of Cu²⁺ with CIP, which guaranteed the sequential detection of Cu²⁺. Meanwhile, the distinct fluorescent color change from bright blue to red, then back to blue, enabled naked-eye visual detection of CIP and Cu²⁺ in the solution phase and on paper-based test strip, and was successfully applied to determine the levels of CIP in complicated food samples with high sensitivity.