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Single-cell Microfluidic Analysis of Bacillus subtilis
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Bioinformatics analysis of extracellular subtilisin E from Bacillus subtilis
Reza Zolfaghari Emameh1, Justina Kazokaitė2, Bagher Yakhchali1
1Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Journal of Biomolecular Structure & Dynamics
|March 5, 2021
Summary
This study used bioinformatics to analyze subtilisin E from Bacillus subtilis, identifying key amino acids and stabilizing mutations. Findings suggest improving subtilisin E production and stability through genetic engineering and transcription factor analysis.
Area of Science:
- Biochemistry
- Molecular Biology
- Bioinformatics
Background:
- Subtilisin E from Bacillus spp. is crucial for biotechnology.
- Its efficacy is influenced by its 3D structure.
Purpose of the Study:
- To evaluate subtilisin E from Bacillus subtilis using bioinformatic methods.
- To identify factors affecting its stability and expression.
Main Methods:
- Bioinformatic analysis of subtilisin E sequence and structure.
- Evaluation of point mutations (N123V, S331L) for stability.
- Genomic analysis of the aprE gene and its regulatory elements.
- Phylogenetic analysis of subtilisin E across Bacillus species.
Main Results:
- Identified conserved amino acids (histidine, aspartic acid, serine) in subtilisin E.
- Determined N123V and S331L mutations as most stabilizing.
- Revealed aprE and yhfN gene expression via a σA promoter.
- Identified key transcription factors (TFs) and transcription factor binding factors (TBFs) regulating aprE.
Conclusions:
- Subtilisin E structures are conserved across Bacillus species.
- Overexpression and stability of subtilisin E can be enhanced.
- System biology approaches, including point mutations and TF/TBF identification, are promising.
