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High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
Published on: June 29, 2018
Escaping the Finnegan - Is it time?
Rachana Singh1, Jonathan M Davis2
1Division of Newborn Medicine, Tufts Children's Hospital, Boston, MA, 02111, USA.
Abstract:
Neonatal Abstinence Syndrome (NAS) has significantly increased worldwide secondary to a marked increase in the incidence of opioid use disorders (OUD) in women of childbearing age. Since first described in 1975, the Finnegan Neonatal Abstinence Scoring Tool (FNAST) remains the mainstay of monitoring NAS severity and its clinical management. The complexity of the tool (21 independent variables), the need for external validation, excessive subjectivity, poor inter-rater reliability, and uncertainty regarding the clinical relevance of some items has resulted in the need to develop an alternate scoring tool. A validated, simple, clinically relevant, and universally accepted approach to assessing opioid exposed neonates would facilitate high quality clinical care while assisting in the generation of generalizable data from future research studies conducted in this vulnerable population.
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