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Complete Genome Sequence of Aeromonas caviae Strain MS6064, a mcr-3-Carrying Clinical Isolate from Japan

Liansheng Yu1,2,3, Hiroki Kitagawa3,4,5, Shizuo Kayama1,2,3

  • 1Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan.

Microbiology Resource Announcements
|March 5, 2021
PubMed

Insights

We sequenced the genome of an Aeromonas caviae strain from a Japanese patient, finding it carries the mcr-3 gene variant 3.38. This strain showed borderline resistance to colistin, an important antibiotic.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • The emergence of colistin resistance is a significant global health concern.
  • The mcr-3 gene confers resistance to colistin, a last-resort antibiotic.
  • Aeromonas species are increasingly recognized as opportunistic pathogens.

Purpose of the Study:

  • To report the complete genome sequence of a mcr-3-carrying Aeromonas caviae strain.
  • To characterize the specific mcr-3 variant present in the strain.
  • To assess the colistin resistance profile of the isolate.

Main Methods:

  • Whole-genome sequencing of Aeromonas caviae strain MS6064.
  • Bioinformatic analysis to identify antimicrobial resistance genes, including mcr-3.
  • Antimicrobial susceptibility testing to determine colistin resistance levels.

Main Results:

  • The complete genome sequence of Aeromonas caviae strain MS6064 was obtained.
  • The strain harbors the mcr-3 variant 3.38.
  • The isolate exhibited borderline resistance to colistin (2 μg/ml).

Conclusions:

  • This study provides valuable genomic data on a mcr-3-positive Aeromonas caviae isolate.
  • The presence of mcr-3 in Aeromonas species highlights the need for surveillance of colistin resistance.
  • Understanding the genetic basis of resistance in Aeromonas is crucial for effective treatment strategies.

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