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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Complete Genome Sequence of Staphylococcus aureus Strain 834, Isolated from a Septic Patient in Japan
Hisaya K Ono1,2, Yasunori Suzuki3, Hiroaki Kubota4
1Laboratory of Zoonoses, Kitasato University School of Veterinary Medicine, Towada, Japan.
Abstract:
Here, we report the complete genome sequence of Staphylococcus aureus strain 834, which was isolated from a septic patient in Japan and showed strong virulence and methicillin resistance. The complete genome consists of a 2,838,668-bp chromosome and a 24,653-bp plasmid. Genome annotation predicts 2,670 coding sequences, 16 rRNAs, and 61 tRNAs.
Insights
We sequenced the complete genome of the virulent and methicillin-resistant *Staphylococcus aureus* strain 834 from a Japanese septic patient. This provides a valuable resource for understanding this dangerous pathogen.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- *Staphylococcus aureus* is a significant human pathogen.
- Methicillin resistance and high virulence contribute to its clinical threat.
- Understanding the genetic basis of these traits is crucial for developing new treatments.
Purpose of the Study:
- To report the complete genome sequence of *Staphylococcus aureus* strain 834.
- To provide a genomic resource for studying virulence and antibiotic resistance in *S. aureus*.
Main Methods:
- Whole-genome sequencing of *Staphylococcus aureus* strain 834.
- Bioinformatic analysis and genome annotation.
Main Results:
- The complete genome sequence of strain 834 was determined.
- The genome comprises a chromosome (2,838,668 bp) and a plasmid (24,653 bp).
- Genome annotation identified 2,670 coding sequences, 16 rRNAs, and 61 tRNAs.
Conclusions:
- The genome sequence of *S. aureus* strain 834 is now available.
- This genomic data can facilitate research into *S. aureus* pathogenesis and resistance mechanisms.
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