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First Report of Xiphinema Diffusum from Pakistan
E Shokoohi1, K Nasira2, E Iqbal2
1Green Biotechnologies Research Centre of Excellence, University of Limpopo, Private Bag X1106, Sovenga 0727, South Africa.
Helminthologia
|March 5, 2021
Summary
This study identified Xiphinema diffusum, a plant-parasitic nematode, in Pakistan for the first time. Morphological and molecular analyses confirmed its presence, aiding in understanding nematode distribution.
Area of Science:
- Nematology
- Plant Pathology
- Molecular Phylogenetics
Background:
- Plant-parasitic nematodes pose significant threats to agriculture globally.
- The Xiphinema americanum species group is known for its widespread distribution and economic impact on crops.
- Limited information exists on nematode fauna in the Gilgit-Baltistan region of Pakistan.
Purpose of the Study:
- To identify and characterize plant-parasitic nematodes associated with peach roots in Gilgit-Baltistan, Pakistan.
- To determine the species of the Xiphinema americanum group found.
- To provide molecular and morphological data for future taxonomic and phylogenetic studies.
Main Methods:
- Soil samples were collected from the rhizosphere of peach (Prunus persica) trees.
- Morphological analysis and morphometrics were conducted on isolated nematodes.
- Molecular analysis involved sequencing of the 18S ribosomal DNA (rDNA) gene.
- Phylogenetic analysis was performed using obtained rDNA sequences.
Main Results:
- A population belonging to the Xiphinema americanum species group was detected.
- Morphological and morphometric data identified the species as Xiphinema diffusum.
- Molecular analysis of 18S rDNA showed 99% similarity to Chinese and Australian X. diffusum populations.
- Phylogenetic analysis confirmed the Pakistani population's close relationship to other X. diffusum lineages.
Conclusions:
- Xiphinema diffusum is reported for the first time in Pakistan.
- The findings contribute to the understanding of nematode distribution in the region.
- This report highlights the importance of continued surveys for plant-parasitic nematodes in Pakistan's agricultural areas.
