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Related Experiment Video

Updated: Nov 15, 2025

Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy
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Cerebrovascular reactivity measured in awake mice using diffuse correlation spectroscopy.

Rowan O Brothers1, Nir Atlas2, Kyle R Cowdrick1

  • 1Emory University and Georgia Institute of Technology, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, Georgia, United States.

Neurophotonics
|March 5, 2021
PubMed
Summary

We developed a repeatable method to measure cerebrovascular reactivity (CVR) in awake mice using acetazolamide (ACZ) and diffuse correlation spectroscopy. This technique allows for reliable assessment of CVR, aiding in disease screening and therapeutic development.

Keywords:
acetazolamidecerebral blood flowcerebrovascular reactivitydiffuse correlation spectroscopymice

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Area of Science:

  • Neuroscience
  • Vascular Biology
  • Medical Imaging

Background:

  • Cerebrovascular reactivity (CVR) is crucial for brain vascular health, but its dysregulation mechanisms and treatments remain unclear.
  • Quantifying CVR in preclinical mouse models is challenging, hindering research into impaired CVR and potential therapies.

Purpose of the Study:

  • To establish a reliable method for assessing CVR in awake, freely behaving mice.
  • To enable the study of CVR dysregulation and the evaluation of therapeutic interventions in a preclinical setting.

Main Methods:

  • Utilized intraperitoneal acetazolamide (ACZ) as a vasoactive stimulus.
  • Employed minimally invasive diffuse correlation spectroscopy (DCS) with a skull-mounted sensor to monitor cerebral blood flow.
  • Assessed CVR by quantifying the relative increase in blood flow post-ACZ administration.

Main Results:

  • Demonstrated consistent CVR measurements across different brain hemispheres and DCS sensor depths (3 and 4.5 mm).
  • Reported low intra- and inter-mouse variability for global CVR (15% and 19%, respectively) and response half-time (24% and 27%, respectively).
  • Indicated a homogenous, whole-brain response to ACZ stimulus.

Conclusions:

  • Presented a repeatable and reliable method for measuring CVR in freely behaving mice.
  • This technique facilitates screening for CVR impairments in disease models.
  • The method supports tracking CVR changes during therapeutic interventions.