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Updated: Nov 15, 2025

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
Published on: March 20, 2021
Normative dataset for plasma cytokines in healthy human adults
Yingkai Li1, John S Yi2, Melissa A Russo1
1Neuromuscular Division, Department of Neurology, Duke University Medical Center, 915 S. LaSalle St. Ext.; Box 2926, SORF Building; Room 124, Durham, NC 27710, United States.
Abstract:
We determined normative data for plasma cytokines established from a cohort of 126 carefully screened healthy adults aged 18 to 64 years. Participants were enrolled to ensure an even age and sex distribution and to include at least 30% non-Caucasians. Plasma cytokines for 18 analytes were tested by multiplex immunoassay. The data are presented by age cohort (18-29 years, 30-39, 40-49, and 50-66), as well as by sex and racial background. This dataset complements published normative ranges of cellular subsets generated by comprehensive polychromatic flow cytometry analysis of the healthy human immune system [1]. These data are available to researchers and have value as a reference range for research involving peripheral cytokines.
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