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Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

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Related Experiment Video

Updated: Nov 15, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

Transcriptomic data showing differentially expressed genes between Notch3 and Notch4 deleted mice.

Madhulika Sharma^1,2, Sireesha Yerrathota^1,2, Mackenzie M Thornton^1,2

¹Departments of Internal Medicine, United States.

|March 5, 2021

Summary

This study compared gene expression in the kidneys of Notch4 and Notch3 mice. RNA sequencing identified differential gene expression, revealing potential targets for Notch signaling in kidney diseases.

Keywords:

Chronic kidney disease HIVAN Inflammation Tg26

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Last Updated: Nov 15, 2025

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Area of Science:

Developmental Biology
Molecular Biology
Genomics

Background:

The Notch signaling pathway is crucial for maintaining homeostasis during development.
While Notch4 and Notch3 gene deletions are not lethal in mice, their specific roles in kidney homeostasis require further investigation.

Purpose of the Study:

To analyze differential gene expression in the kidneys of Notch4 knockout mice compared to Notch3 knockout mice.
To identify potential gene targets regulated by Notch3 and Notch4 signaling in the kidney.

Main Methods:

Kidneys were collected from 3-month-old Notch4 and Notch3 knockout littermate mice on an FVB background.
RNA sequencing (RNA-seq) was performed on kidney tissue.
Differential gene expression analysis was conducted using DeSeq2 software with a P-value threshold of ≤0.05 and an absolute fold change of ≥1.5.

Main Results:

RNA sequencing revealed significant differences in gene expression between Notch4 and Notch3 knockout mouse kidneys.
Specific upregulated and downregulated genes were identified in Notch4 knockout mice relative to Notch3 knockout mice.
The analysis provides a dataset of genes potentially regulated by Notch3 and Notch4 signaling.

Conclusions:

The study identifies differentially expressed genes in the kidneys of Notch4 and Notch3 knockout mice.
These findings offer insights into the distinct roles of Notch3 and Notch4 in kidney physiology.
The identified genes serve as potential targets for understanding and treating kidney diseases involving aberrant Notch3 or Notch4 expression.