PASTA: PARP activity screening and inhibitor testing assay
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Updated: Nov 15, 2025
Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
Published on: March 3, 2015
Ilsa T Kirby1, Daniel J Sanderson2, Michael S Cohen2
1University of California, San Francisco, Department of Cellular and Molecular Pharmacology, San Francisco, CA 94117, USA.
Abstract:
Small-molecule inhibitors have been instrumental in uncovering the biological importance of poly-ADP-ribose polymerases (PARPs), a family of enzymes involved in wide-ranging aspects of cell biology. However, few PARP inhibitors are tested against the entire family of PARPs. This makes it impossible to confidently assess the role of a single PARP in cellular processes using small molecules. Here, we detail a PARP activity screening and inhibitor testing assay (PASTA) for determining relative selectivity of PARP inhibitors. For complete details on the use and execution of this protocol, please refer to Kirby et al. (2018).
