Rhodohalobacter mucosus sp. nov., isolated from a marine solar saltern

A Gram-stain-negative, non-motile, aerobic, rod-shaped and reddish-pigmented bacterium, designated 8A47^T, was isolated from a marine solar saltern located in Wendeng, PR China. The novel strain 8A47^T grows at 20-42 °C, pH 7.0-9.0, and in the presence of 2.0-14.0% (w/v) NaCl. Optimal growth was observed at 37-40 °C, pH 7.5-8.0, and with 4.0-6.0% (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 8A47^T formed an evolutionary lineage with members of the genus Rhodohalobacter. Strain 8A47^T exhibited high level of similarity to Rhodohalobacter barlenses MCCC 1K03442^T (94.7%) and Rhodohalobacter halophilus JZ3C29^T (93.5%). The major fatty acids were summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), C_16:0 and iso-C_15:0. The sole respiratory quinone was MK-7. The polar lipids of the new isolate consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid, two unidentified phospholipids and three unidentified glycolipids and. The genomic DNA G + C content of the strain 8A47^T was 47.7 mol%. Based on its phenotypic, chemotaxonomic, genotypic and genomic characteristics presented in this study, strain 8A47^T is considered to represent a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter mucosus sp. nov. is proposed. The type strain is 8A47^T (= KCTC 62603^T = MCCC 1H00329^T).