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Isothermal titration calorimetry (ITC): a standard operating procedure (SOP)
Margarida Bastos1, Adrian Velazquez-Campoy2,3,4,5
1CIQ-UP, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Porto, Portugal. mbastos@fc.up.pt.
European Biophysics Journal : EBJ
|March 5, 2021
Summary
This study presents a Standard Operating Procedure (SOP) for Isothermal Titration Calorimetry (ITC) to ensure scientifically correct and comparable results in biophysical research. The SOP addresses experimental design and machine calibration for reliable data.
Area of Science:
- Biophysical Chemistry
- Biomolecular Interactions
- Analytical Chemistry
Background:
- Isothermal Titration Calorimetry (ITC) is a widely used technique for studying molecular interactions.
- Existing research spans diverse fields including protein-ligand binding, DNA interactions, and polymer surfactant studies.
- A need exists for standardized protocols to ensure data accuracy and comparability across different laboratories.
Purpose of the Study:
- To establish a comprehensive Standard Operating Procedure (SOP) for Isothermal Titration Calorimetry (ITC).
- To provide guidelines for scientifically correct and comparable experimental results in ITC studies.
- To support the ARBRE-MOBIEU COST Action (CA15126) by developing standardized biophysical techniques.
Main Methods:
- Development of an ITC Standard Operating Procedure (SOP) by Working Group 4 of the ARBRE-MOBIEU COST Action.
- Discussion of critical factors for effective experimental design in ITC.
- Emphasis on machine calibration, including chemical calibration, for accurate measurements.
Main Results:
- A detailed ITC SOP has been generated, addressing key aspects of experimental setup and execution.
- Factors crucial for robust experimental design and accurate machine calibration are outlined.
- The SOP aims to enhance the reliability and reproducibility of ITC data.
Conclusions:
- The developed ITC SOP is essential for users seeking scientifically sound and comparable data.
- Adherence to the SOP, including proper calibration, will improve the quality of ITC research.
- This work contributes to the standardization of biophysical techniques within the scientific community.