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Dither removing Fourier ptychographic microscope based on a two-axis rotation stage.
Kaicheng Huang1, Wangwei Hui1, Qing Ye1
1Ministry of Education, Nankai University, School of Physics, Key Laboratory of Weak-Light Nonlinear, China.
Journal of Biomedical Optics
|March 5, 2021
Summary
A novel motorized illuminator enhances Fourier ptychographic microscopy (FPM) flexibility for diverse biomedical imaging needs. This system offers adaptable illumination and a dither removal algorithm, improving imaging resolution and quality.
Area of Science:
- Optical microscopy
- Computational imaging
- Biomedical imaging
Background:
- Fourier ptychographic microscopy (FPM) significantly enhances microscope space-bandwidth product.
- Current FPM illuminators lack flexibility, hindering adaptation to varied imaging requirements.
Purpose of the Study:
- To develop a more flexible illuminator for FPM to meet diverse imaging needs.
- To introduce a dither removal algorithm for improved image acquisition in FPM.
Main Methods:
- A two-axis motorized rotation stage was employed for a flexible illuminator design.
- A concentric illumination method was adopted for variable directional coherent illumination.
- A dither removing algorithm was developed to mitigate image acquisition artifacts.
Main Results:
- The illuminator demonstrated successful integration into systems with numerical apertures of 0.045 and 0.01.
- Resolution gains of approximately 4-fold and 13-fold were achieved, respectively.
- The dither removing algorithm's effectiveness was confirmed through simulations and experiments.
Conclusions:
- A flexible illuminator for FPM was successfully presented, addressing the need for adaptable imaging.
- The proposed dither removing algorithm effectively reduces artifacts, enhancing FPM performance.
