Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 15, 2025

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.0K

iTRAQ-based comparative proteomic analysis in different developmental stages of Echinococcus granulosus.

Xin Li1, Song Jiang2, Xuhai Wang2

  • 1College of Life Sciences, Shihezi University, Road Beisi, Shihezi 832003, Xinjiang, PR China - College of Animal Science and Technology, Shihezi University, Road Beisi, Shihezi 832003, Xinjiang, PR China.

Parasite (Paris, France)
|March 5, 2021
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Bacterial and Fungal Community Structure and Potential Function Analysis of Yak Feces before and after Weaning.

BioMed research international·2022
Same author

Intestinal transcriptomes in Kazakh sheep with different haplotypes after experimental Echinococcus granulosus infection.

Parasite (Paris, France)·2021
Same author

The Use of RNAi Technology to Interfere with <i>Zfx</i> Gene Increases the Male Rates of Red Deer (<i>Cervus elaphus</i>) Offspring.

BioMed research international·2020
See all related articles

Researchers identified stage-specific proteins in Echinococcus granulosus, the parasite causing cystic echinococcosis. These proteins, particularly those highly expressed in the oncosphere stage, show promise as vaccine targets for preventing this chronic zoonotic disease.

Area of Science:

  • Parasitology
  • Molecular Biology
  • Immunology

Background:

  • Cystic echinococcosis is a chronic zoonotic disease caused by Echinococcus granulosus.
  • The parasite's lifecycle involves distinct stages requiring specific gene and protein expression for survival.
  • Developing effective vaccines requires understanding stage-specific antigens.

Purpose of the Study:

  • To screen and analyze stage-differential antigens of Echinococcus granulosus.
  • To identify potential targets for vaccine development against E. granulosus.
  • To investigate proteins crucial for parasite survival across different life stages.

Main Methods:

  • Utilized isobaric tags for relative and absolute quantification (iTRAQ) to identify differentially expressed proteins.
Keywords:
Candidate targets for diagnosis or therapeuticsDifferential expression proteinsE. granulosusiTRAQ

More Related Videos

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

10.7K
Transient Transduction of the Strobilated Forms of Echinococcus granulosus
13:25

Transient Transduction of the Strobilated Forms of Echinococcus granulosus

Published on: September 16, 2022

3.3K

Related Experiment Videos

Last Updated: Nov 15, 2025

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.0K
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

10.7K
Transient Transduction of the Strobilated Forms of Echinococcus granulosus
13:25

Transient Transduction of the Strobilated Forms of Echinococcus granulosus

Published on: September 16, 2022

3.3K
  • Analyzed protein expression across three key life stages: oncosphere, adult tapeworm, and protoscolex.
  • Performed bioinformatics analysis including COG, GO, and metabolic pathway annotation.

    • Main Results:

    • Identified a significant number of differentially expressed proteins between life stages (375: oncosphere vs. adult, 346: oncosphere vs. protoscolex, 391: adult vs. protoscolex).
    • Differential proteins were notably enriched in metabolic, neurodegenerative disease-related (Huntington's, Alzheimer's), and ribosome pathways.
    • Paramyosin, HSP60/70/90, cathepsins L1/D, casein kinase, and calmodulin showed significantly higher expression in the oncosphere stage (p < 0.05).

    Conclusions:

    • The study identified stage-specific proteins in Echinococcus granulosus with potential for vaccine development.
    • Proteins highly expressed in the oncosphere stage, such as paramyosin and heat shock proteins, are promising candidates for diagnostic and prophylactic interventions.
    • These findings contribute to understanding parasite biology and developing strategies against cystic echinococcosis.