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Updated: Jun 16, 2026
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in
Stéphanie Miserey-Lenkei1, Katarina Trajkovic2, Juan Martín D'Ambrosio3
1Department of Cell Biology and Cancer, Institut Curie, PSL Research University, Sorbonne Université, CNRS, UMR144, Paris, F-75005, France.
Background Information:
Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins.
Results:
We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria.
Conclusions And Significance:
This library should facilitate further cellular investigations, notably by imaging techniques.
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