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Related Concept Videos

Leaky Scanning02:28

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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
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NMD-mediated control of Tor influences adaptation to nutrient and temperature conditions in <i>Cryptococcus neoformans</i>.

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Correction: Thaochan et al. Fungal-Infected Weeds: A Potential Source of Leaf Spot Disease in Rubber Trees from Southern Thailand. <i>J. Fungi</i> 2025, <i>11</i>, 220.

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Tools for Assessing Translation in Cryptococcus neoformans.

Corey M Knowles1, Kelcy M McIntyre1, John C Panepinto1

  • 1Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY 14201, USA.

Journal of Fungi (Basel, Switzerland)
|March 6, 2021
PubMed
Summary
This summary is machine-generated.

Cryptococcus neoformans combats cellular stress via translatome reprogramming. Understanding translation regulation is key for developing new antifungal drugs targeting this fungus.

Keywords:
elongationhost adaptationinitiationrepressiontranslationtranslation regulation

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Area of Science:

  • Mycology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Cryptococcus neoformans is a fungus found in the environment that can infect humans.
  • Host immune system interactions and environmental changes induce significant cellular stress in C. neoformans.
  • Translatome reprogramming, specifically translation repression, is a key mechanism for managing cellular stress and reallocating resources.

Purpose of the Study:

  • To review the mechanisms of translation regulation in C. neoformans during host infection.
  • To highlight the importance of understanding translational control for antifungal drug development.
  • To discuss tools for assessing translation states and output under cellular stress.

Main Methods:

  • Review of existing literature on C. neoformans stress response and translation.
  • Discussion of methodologies for analyzing translatome changes.
  • Examination of techniques to measure translational output.

Main Results:

  • Cellular stress in C. neoformans triggers translatome reprogramming.
  • Translation repression is a conserved stress response mechanism.
  • Specific tools exist to study these dynamic changes in gene expression.

Conclusions:

  • Understanding translation regulation in C. neoformans is critical for developing novel antifungal therapies.
  • Assessing changes in translation state and output provides insights into fungal adaptation and virulence.
  • Further research into these mechanisms can guide the development of targeted interventions against cryptococcal infections.