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Related Experiment Videos

Enrichment for Plesiomonas shigelloides from stools.

Z Rahim1, B A Kay

  • 1International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.

Journal of Clinical Microbiology
|April 1, 1988
PubMed
Summary

Bile peptone broth significantly improves Plesiomonas shigelloides isolation from stool samples. This method is more effective than direct plating and alkaline peptone water for detecting this bacterium.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Bacteriology

Background:

  • Plesiomonas shigelloides is an emerging enteric pathogen.
  • Accurate isolation of P. shigelloides from clinical samples is crucial for diagnosis and treatment.

Purpose of the Study:

  • To evaluate the efficacy of bile peptone broth as an enrichment medium for Plesiomonas shigelloides isolation.
  • To compare bile peptone broth with alkaline peptone water and direct plating methods.

Main Methods:

  • Stool samples were analyzed using parallel examinations.
  • Enrichment was performed using bile peptone broth and alkaline peptone water.
  • Isolation was conducted on salmonella-shigella agar.

Main Results:

  • Bile peptone broth enrichment for 24 hours was six times more effective than direct plating (P < 0.01).
  • Bile peptone broth demonstrated twice the recovery rate of P. shigelloides compared to alkaline peptone water.

Conclusions:

  • Bile peptone broth is a highly effective enrichment medium for isolating Plesiomonas shigelloides from stool.
  • This method offers a significant improvement over direct plating and alkaline peptone water for P. shigelloides detection.

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