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Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template.
Gerard Rubí-Sans1,2, Irene Cano-Torres1,2, Soledad Pérez-Amodio1,2,3
1Biomaterials for Regenerative Therapies Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain.
Biomedicines
|March 6, 2021
Summary
Researchers developed cell-derived matrix microtissues using specific scaffolds and stem cells. These engineered tissues mimic natural environments and show potential for vascularization in regenerative medicine.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Current tissue engineering strategies struggle to replicate the native extracellular matrix (ECM) environment.
- Mimicking the complex physical and biochemical properties of native tissues is crucial for successful regeneration.
- Organ transplantation remains a significant challenge due to donor shortages and immune rejection.
Purpose of the Study:
- To develop a novel method for fabricating cell-derived matrix (CDM) microtissues (MTs).
- To create microtissues that effectively mimic the native tissue microenvironment.
- To assess the angiogenic potential of the fabricated microtissues for tissue vascularization.
Main Methods:
- Utilized poly-lactic acid (PLA) and Cultispher® S microcarriers (MCs) as scaffold templates.
- Seeded scaffolds with rat bone marrow mesenchymal stem cells (rBM-MSCs) to facilitate ECM deposition.
- Extracted the newly formed CDM from the scaffold templates for downstream applications.
Main Results:
- The fabricated CDM microtissues provided a complex physical and biochemical environment for cells.
- Spontaneous angiogenic potential was observed in the developed microtissues.
- Mixed microtissues, generated from a combination of PLA and Cultispher® S MCs, demonstrated excellent potential for tissue vascularization.
Conclusions:
- The developed methodology enables in-house fabrication of microtissues with inherent angiogenic properties.
- These cell-derived matrix microtissues are promising candidates for enhancing vascularization in tissue regenerative strategies.
- This approach offers a viable alternative to organ transplantation by promoting functional tissue regeneration.
