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A Bacterially-Expressed Recombinant Envelope Protein from Usutu Virus Induces Neutralizing Antibodies in Rabbits.

Kinga Böszörményi1, Janet Hirsch2, Gwendoline Kiemenyi Kayere1

  • 1Department of Virology, Biomedical Primate Research Centre, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands.

Vaccines
|March 6, 2021
PubMed
Summary

Researchers produced a partial Usutu virus (USUV) envelope protein in E. coli. Immunization generated neutralizing antibodies, paving the way for a potential USUV subunit vaccine.

Keywords:
USUVUsutu virusemerging diseaseflavivirusneutralizing antibodiesrecombinant E proteinvaccine development

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Area of Science:

  • Virology
  • Immunology
  • Vaccine Development

Background:

  • Usutu virus (USUV), an emerging flavivirus, has caused significant bird die-offs in Europe.
  • USUV is spreading geographically, posing a potential threat to human health, similar to West Nile and Zika viruses.
  • Developing an effective vaccine against USUV is crucial due to its expanding reach.

Purpose of the Study:

  • To produce and purify a partial Usutu virus envelope protein for vaccine research.
  • To assess the immunogenicity of the refolded Usutu virus envelope protein.

Main Methods:

  • Production of a partial Usutu virus (USUV) envelope protein in E. coli.
  • Purification and refolding of the USUV envelope protein.
  • Characterization of the protein using size exclusion chromatography, TEM, and DLS.

Main Results:

  • The refolded protein solution contained a mixture of mono and multimeric envelope proteins.
  • Immunization of rabbits with the refolded E-protein and adjuvant generated neutralizing antibodies.
  • In vitro assays confirmed the presence of neutralizing antibodies against Usutu virus.

Conclusions:

  • The study successfully produced a partial Usutu virus envelope protein.
  • The generated neutralizing antibodies indicate potential for a subunit vaccine.
  • Further research is needed to develop a fully effective Usutu virus vaccine.