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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: Nov 15, 2025

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

Oligonucleotide-Based Approaches to Inhibit Dengue Virus Replication.

Kingshuk Panda¹, Kalichamy Alagarasu¹, Deepti Parashar¹

¹Dengue & Chikungunya Group, ICMR-National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411001, India.

Molecules (Basel, Switzerland)

|March 6, 2021

Summary

Developing new therapies is crucial as no vaccines or antivirals exist for Dengue virus (DENV). Oligonucleotide strategies offer a promising approach to inhibit DENV replication by targeting viral RNA, potentially reducing disease severity.

Keywords:

RNA interference antisense oligonucleotides antiviral dengue ribozymes

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Last Updated: Nov 15, 2025

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

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Published on: November 1, 2018

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Published on: May 5, 2014

High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication

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Published on: October 30, 2021

Area of Science:

Virology
Molecular Biology
Public Health

Background:

Dengue virus (DENV) infection is a significant and growing global health concern, particularly in tropical and subtropical areas.
Current treatment options for Dengue fever are limited, with no effective vaccines or antiviral drugs available.
There is an urgent need for novel therapeutic strategies to mitigate the impact of this severe disease.

Purpose of the Study:

This review explores oligonucleotide-based therapeutic strategies for inhibiting Dengue virus replication.
The study aims to discuss various approaches, including ribozymes, RNA interference, CRISPR, aptamers, and morpholinos.
The objective is to evaluate the potential of these methods in combating Dengue virus infection.

Main Methods:

The review synthesizes existing research on oligonucleotide-based antiviral strategies.
It specifically examines methods targeting viral RNA to inhibit replication.
Approaches discussed include ribozymes, RNA interference (RNAi), CRISPR systems, aptamers, and morpholinos.

Main Results:

Oligonucleotide strategies can be designed to specifically target and degrade Dengue virus RNA.
This targeted RNA destruction effectively inhibits viral replication.
These methods show potential for interfering with viral processes without negatively impacting host cellular functions.

Conclusions:

Oligonucleotide-based approaches represent a promising avenue for developing new Dengue virus therapies.
Strategies like RNA interference, CRISPR, aptamers, and morpholinos offer targeted inhibition of viral replication.
Further research and development are necessary to overcome challenges and translate these findings into clinical applications for Dengue fever treatment.