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Related Experiment Video

Updated: Nov 15, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

Rapid Methods for Antimicrobial Resistance Diagnostics.

Georgia D Kaprou^1,2, Ieva Bergšpica^1,3, Elena A Alexa¹

¹Department of Food Hygiene and Technology, University of León, 24071 León, Spain.

Antibiotics (Basel, Switzerland)

|March 6, 2021

Summary

This summary is machine-generated.

Antimicrobial resistance (AMR) demands faster diagnostics. This review explores advanced antimicrobial susceptibility testing (AST) methods, including sequencing and spectroscopy, to combat this global health threat.

Keywords:

FTIR MALDI-TOF antibiotic susceptibility testing antimicrobial resistance lab-on-a-chip microfluidics molecular diagnostics point-of-care sequencing

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Area of Science:

Microbiology
Public Health
Biotechnology

Background:

Antimicrobial resistance (AMR) poses a significant global public health challenge.
Conventional antimicrobial susceptibility testing (AST) methods are time-consuming, costly, and contribute to the spread of AMR.
Rapid diagnostics are crucial for effective treatment and containment of AMR.

Purpose of the Study:

To review current and emerging technologies for rapid antimicrobial susceptibility testing (AST).
To highlight advancements in AMR diagnostics and their potential impact on clinical microbiology.
To discuss the implementation of novel diagnostic strategies for AMR.

Main Methods:

Review of conventional AMR detection and AST methods.
Examination of non-conventional methods: whole genome sequencing (WGS), MALDI-TOF, FTIR spectroscopy, and microfluidics.
Analysis of commercially available diagnostic platforms for AST.

Main Results:

Conventional methods for AMR diagnostics are often slow and expensive.
Emerging technologies like WGS, MALDI-TOF, FTIR, and microfluidics offer faster and potentially more accurate AST.
Several commercial platforms are available, with ongoing development in diagnostic technologies.

Conclusions:

Advanced diagnostic tools are essential to combat the growing threat of AMR.
Technological innovations in AMR diagnostics can significantly improve patient outcomes and reduce healthcare costs.
Further development and implementation of rapid AST methods are critical for global health security.