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Updated: Nov 15, 2025
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Cell-Free DNA Integrity Analysis in Urine Samples
Published on: January 5, 2017
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Urinary Cell-Free DNA in Bladder Cancer Detection
Ryan Tsz-Hei Tse1, Hongda Zhao1, Christine Yim-Ping Wong1
1S.H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong, China.
Diagnostics (Basel, Switzerland)
|March 6, 2021
Summary
Urinary cell-free DNA (ucfDNA) shows promise for detecting and classifying bladder cancer. Analyzing ucfDNA molecular and genetic features offers a less invasive alternative to cystoscopy for bladder cancer surveillance.
Area of Science:
- Urology
- Molecular Biology
- Oncology
Background:
- Urinary bladder cancer is a prevalent urological malignancy.
- Flexible cystoscopy, a common diagnostic tool, is invasive, costly, and uncomfortable.
- Urinary cell-free DNA (ucfDNA) presents a promising non-invasive biomarker for bladder cancer detection and surveillance.
Purpose of the Study:
- To evaluate the potential of ucfDNA in bladder cancer detection and surveillance.
- To explore the utility of ucfDNA molecular features for differentiating bladder cancer patients from healthy individuals.
- To investigate the application of ucfDNA in molecular classification of bladder cancer subtypes.
Main Methods:
- Isolation and analysis of ucfDNA from urine supernatant.
- Assessment of ucfDNA integrity and concentration.
- Sequencing and expression analysis of ucfDNA for genetic profiling.
Main Results:
- ucfDNA integrity and concentration effectively differentiate bladder cancer patients from healthy controls.
- Distinct molecular and genetic features of ucfDNA are identifiable in bladder cancer.
- Significant molecular and genetic differences in ucfDNA are observed between non-muscle-invasive and muscle-invasive bladder cancers.
Conclusions:
- ucfDNA is a highly promising tool for non-invasive bladder cancer detection and surveillance.
- ucfDNA analysis can aid in the molecular classification of bladder cancer.
- Future applications of ucfDNA in bladder cancer management are anticipated.
