Biocatalytic C-C Bond Formation for One Carbon Resource Utilization

Area of Science:

• Biocatalysis and Synthetic Biology
• Green Chemistry
• C1 Resource Utilization

Background:

• Carbon-carbon bond formation is crucial for C1 resource utilization.
• Biocatalysis presents a sustainable alternative to traditional organic synthesis for C1 transformation.
• Advances in synthetic biology have enabled the discovery and design of novel enzymes for C1 chemistry.

Purpose of the Study:

• To provide an overview of biocatalytic C-C bond formation in C1 resource utilization.
• To review newly mined and designed carboxylases and C-C ligases for C1 transformation.
• To discuss catalytic mechanisms and future perspectives in this field.

Main Methods:

• Literature review of biocatalytic C-C bond formation strategies.
• Analysis of newly discovered and engineered carboxylases and ligases.
• Discussion of enzymatic mechanisms for C1 feedstock conversion.

Main Results:

• Overview of biocatalytic C-C bond formation in C1 utilization.
• Detailed review of carboxylases and ligases for CO2, formaldehyde, CO, and formate transformation.
• Discussion on catalytic mechanisms of these enzymes.

Conclusions:

• Biocatalysis is a powerful approach for sustainable C1 resource utilization.
• Engineered enzymes show significant potential for efficient C1 transformation.
• Further development of catalysts is crucial for advancing C1 resource applications.