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Candidate Glycoprotein Biomarkers for Canine Visceral Hemangiosarcoma and Validation Using Semi-Quantitative
Patharee Oungsakul1,2, Eunju Choi2,3, Alok K Shah2,4
1School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia.
Veterinary Sciences
|March 6, 2021
Summary
Researchers identified novel serum glycoprotein biomarkers for canine visceral hemangiosarcoma (HSA) using lectin magnetic bead array-coupled tandem mass spectrometry (LeMBA-MS/MS). A combination of Datura stramonium (DSA) lectin histochemistry and complement C7 immunohistochemistry (IHC) shows promise for improved canine HSA diagnosis.
Area of Science:
- Veterinary Oncology
- Biomarker Discovery
- Proteomics
Background:
- Visceral hemangiosarcoma (HSA) is a common and aggressive cancer in dogs, often diagnosed late, leading to poor prognosis.
- Early and differential diagnosis of canine HSA requires reliable point-of-care and prognostic biomarkers, preferably from minimally invasive samples.
- Current diagnostic methods for HSA lack specificity and sensitivity, necessitating the development of novel diagnostic tools.
Purpose of the Study:
- To discover and validate serum glycoprotein biomarkers for early and differential diagnosis of canine visceral hemangiosarcoma (HSA).
- To identify lectins capable of capturing relevant glycoproteins from canine serum for biomarker discovery.
- To validate the diagnostic potential of identified biomarkers in canine HSA tissues.
Main Methods:
- Application of a lectin magnetic bead array-coupled tandem mass spectrometry (LeMBA-MS/MS) workflow in both discovery and validation phases.
- Screening of serum glycoprotein candidates using Datura stramonium (DSA), wheat germ agglutinin (WGA), Sambucus nigra (SNA), and Pisum sativum (PSA) lectins.
- Independent validation of serum LeMBA-MS/MS findings using lectin-histochemistry and immunohistochemistry (IHC) on canine HSA tissues.
Main Results:
- The LeMBA-MS/MS approach identified numerous validated candidate serum glycoproteins.
- DSA, WGA, SNA, and PSA lectins captured the highest number of validated candidate glycoproteins.
- Combination of DSA histochemistry and complement C7 IHC on tumor tissues demonstrated a strong correlation with HSA, enhancing diagnostic specificity.
Conclusions:
- Serum glycoprotein profiling using LeMBA-MS/MS is a viable strategy for discovering canine HSA biomarkers.
- Specific lectins like DSA can effectively capture relevant glycoproteins from canine serum.
- A combined approach using DSA histochemistry and complement C7 IHC offers a promising avenue for more specific diagnosis of canine visceral hemangiosarcoma.
