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Updated: Nov 15, 2025

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Published on: August 17, 2015
Signal amplification strategies for clinical biomarker quantification using elemental mass spectrometry
Andrea L Larraga-Urdaz1, Maria L Fernandez Sanchez1, Jorge Ruiz Encinar2
1Department of Physical and Analytical Chemistry, University of Oviedo, Avda. Julian Claveria, 8, 33006, Oviedo, Spain.
Abstract:
The current trends in modern medicine towards early diagnosis, or even prognosis, of different diseases have brought about the need for the corresponding biomarker detection at ever lower levels in really complex matrices. To do so, it is necessary to use proper extremely sensitive detection techniques such as elemental mass spectrometry. However, target labelling with metals for subsequent sensitive ICP-MS detection falls short nowadays even if resorting to inorganic nanoparticles containing a high number of detectable elements. Thus, new amplification strategies are being proposed to face this analytical challenge that will be critically discussed in this paper. Fundamentals of different novel strategies developed to achieve signal amplification and sensitive elemental mass spectrometry detection are here discussed. Some representative examples of relevant clinical applications are highlighted, along with future prospects and challenges.
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