Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell rubidium uptake: a method for studying functional heterogeneity in the nephron.

F X Beck1, A Dörge, E Blümner

  • 1Department of Physiology, University of Munich, Federal Republic of Germany.

Kidney International
|March 1, 1988
PubMed
Summary

Rubidium uptake reveals distinct potassium transport activity across rat kidney tubule cells. Distal tubule and principal cells show higher rubidium uptake, correlating with their known ion transport functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combination of B cell biomarkers as independent predictors of response in patients with rheumatoid arthritis treated with rituximab.

Clinical and experimental rheumatology·2015
Same author

Some electrical properties of single renal tubule cells.

The Journal of general physiology·2009
Same author

Torasemide significantly reduces thiazide-induced potassium and magnesium loss despite supra-additive natriuresis.

European journal of clinical pharmacology·2009
Same author

Renal and extrarenal regulation of potassium.

Kidney international·2007
Same author

Maxi-K channels contribute to urinary potassium excretion in the ROMK-deficient mouse model of Type II Bartter's syndrome and in adaptation to a high-K diet.

Kidney international·2006
Same author

Ammonium affects tight junctions and the cytoskeleton in MDCK cells.

Pflugers Archiv : European journal of physiology·2005

Area of Science:

  • Nephrology
  • Renal Physiology
  • Cellular Transport

Background:

  • Potassium transport is crucial for renal function.
  • Understanding cell-specific ion transport is key to renal physiology.
  • Rubidium serves as a useful analog for potassium in transport studies.

Purpose of the Study:

  • To investigate and quantify rubidium uptake in individual rat renal tubule cells.
  • To correlate rubidium uptake with known sodium and potassium transport functions in different nephron segments.
  • To differentiate transport activity between various renal tubule cell types.

Main Methods:

  • Utilized energy-dispersive X-ray microanalysis on freeze-dried cryosections of rat renal cortex.
  • Measured rubidium content in individual tubule cells following intravenous rubidium infusion.

Related Experiment Videos

  • Stimulated distal sodium and potassium transport using hypertonic saline loading to assess rubidium uptake changes.

    • Main Results:

    • Rubidium uptake increased in all tubule cells within 30 seconds of infusion.
    • Significant differences in rubidium content were observed: distal convoluted tubule (225 +/- 8), connecting tubule (156 +/- 7), principal cells (110 +/- 7), proximal tubule (86 +/- 4), and intercalated cells (24 +/- 2) (mmol/kg dry wt).
    • Hypertonic saline loading selectively increased rubidium uptake in distal convoluted tubule cells (+38%), connecting tubule cells (+36%), and principal cells (+52%), while proximal tubule and intercalated cells showed no change.

    Conclusions:

    • Preferential rubidium uptake in distal convoluted tubule, connecting tubule, and principal cells aligns with their established roles in sodium and potassium transport.
    • Intercalated cells exhibit low sodium and potassium transport activity, as indicated by minimal rubidium uptake.
    • This study provides cellular-level insights into regional differences in ion transport within the rat kidney.