An ultrasensitive heart-failure BNP biosensor using B/N co-doped graphene oxide gel FET

Area of Science:

• Biomedical Engineering
• Materials Science
• Nanotechnology

Background:

• Heart failure (HF) is a leading global cause of mortality.
• B-type natriuretic peptide (BNP) is a key biomarker for HF diagnosis.
• Existing FET biosensors for BNP have limitations in sensitivity and detection range.

Purpose of the Study:

• To develop a highly sensitive and rapid FET biosensor for BNP detection.
• To utilize B/N co-doped graphene oxide (BN-GO) gel as the channel material.
• To demonstrate the potential of BN-GO FETs for early HF detection.

Main Methods:

• Fabrication of a field-effect transistor (FET) biosensor using BN-GO gel as the channel.
• Testing the biosensor's response to varying concentrations of BNP.
• Evaluating the sensor's selectivity against interfering substances like K+, OH-, and HER2.

Main Results:

• The BN-GO FET biosensor detected BNP in as little as 2 minutes.
• Achieved an ultra-low limit of detection (LOD) of 10 aM.
• Demonstrated a wide linear detection range of 10 aM to 1 μM (11 orders of magnitude).
• Exhibited high selectivity and minimal interference from other biomolecules and ions.

Conclusions:

• BN-GO gel FETs are a promising platform for ultrasensitive biosensing.
• This biosensor offers a significant advancement over current methods for BNP detection.
• The developed biosensor has the potential for early and accurate diagnosis of heart failure.