Impacts of Ag and Ag₂S nanoparticles on the nitrogen removal within vertical flow constructed wetlands treating...

In this study, the effects of silver (Ag NPs) and sliver sulfide nanoparticles (Ag₂S NPs) on nitrogen removal and nitrogen functional microbes in constructed wetlands were investigated. The obtained results demonstrated that inhibition extent on nitrogen removal relied on NPs types and high concentrations NPs showed higher negative effects. 0.5 mg/L Ag NPs had no influence on NH₄⁺-N removal, amoA and nxrA gene copies, whereas Ag₂S NPs and Ag⁺ decreased NH₄⁺-N removal by reducing abundances of nitrifying genes. The concentrations of NO₃^--N and TN in all 0.5 mg/L obviously increased compared with control, resulting from decreasing functional genes and denitrifying bacteria. And 0.5 mg/L Ag NPs exhibited largest inhibitory effects, with the highest NO₃^--N effluent concentrations. 2 mg/L Ag NPs decreased NH₄⁺-N removal, but adverse effects gradually vanished with extension of time, whereas both Ag₂S NPs and Ag⁺ at 2 mg/L influenced NH₄⁺-N transformation and decreased the abundance of amoA and nxrA genes and the AOB Nitrosomonas in CWs. Moreover, 2 mg/L of Ag NPs reduced NO₃^--N removal by decreasing abundance of nirS and key denitrifying bacteria. To sum up, the inhibition mechanisms concluded from current results were possibly in that Ag NPs exhibited nanotoxicity rather than ionic toxicity.