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Enzyme-Linked Immunosorbent Assay01:33

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Aptasensors for lysozyme detection: Recent advances.

Gheorghe Melinte1, Giulia Selvolini2, Cecilia Cristea3

  • 1"Ugo Schiff" Chemistry Department, University of Florence, Via della Lastruccia 3, Sesto Fiorentino (FI), 50019, Italy; Analytical Chemistry Department, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Strada Louis Pasteur 4, Cluj-Napoca, 400349, Romania.

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|March 7, 2021
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Summary

Lysozyme, the body's natural antibiotic, can cause allergies. This review details advanced electrochemical and optical aptasensors for sensitive lysozyme detection in foods, overcoming traditional method limitations.

Keywords:
AllergensAptamerElectrochemical sensorLysozymeOptical sensor

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biosensing Technology

Background:

  • Lysozyme is a crucial enzyme with antibacterial properties, often termed the "body's own antibiotic."
  • However, lysozyme can induce allergic reactions, necessitating accurate quantification in food products.
  • Existing detection methods are often costly, slow, and unsuitable for rapid, in-situ analysis.

Purpose of the Study:

  • To review recent advancements in lysozyme determination using electrochemical and optical aptasensors.
  • To critically evaluate aptamer immobilization techniques and signal amplification strategies.
  • To compare the analytical performance and real-world applicability of various aptasensor designs.

Main Methods:

  • Development and application of electrochemical aptasensors for lysozyme detection.
  • Utilization of optical aptasensors for sensitive lysozyme quantification.
  • Investigation of aptamer immobilization and signal amplification methodologies.
  • Comparative analysis of sensor performance across different platforms.

Main Results:

  • Aptasensors offer enhanced selectivity and sensitivity for lysozyme determination compared to traditional methods.
  • Various aptamer immobilization and signal amplification strategies have been successfully employed.
  • Electrochemical and optical aptasensors demonstrate promising analytical performance for food sample analysis.

Conclusions:

  • Electrochemical and optical aptasensors represent a significant advancement for sensitive and rapid lysozyme detection.
  • These biosensors overcome limitations of conventional methods, enabling efficient food safety monitoring.
  • Further research into aptasensor optimization will enhance their utility in diverse applications.