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Updated: Nov 15, 2025
09:07
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
6.6K
A novel transcript variant of human G-protein coupled estrogen receptor
Uttariya Pal1, Mohan C Manjegowda1, Anil Mukund Limaye2
1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Molecular Biology Reports
|March 7, 2021
Summary
A novel G-protein coupled estrogen receptor variant (GPER-v5) has been identified and confirmed to be expressed in human cancer cell lines. Further research is needed to explore its role in various conditions.
Area of Science:
- Molecular Biology
- Genetics
- Endocrinology
Background:
- The G-protein coupled estrogen receptor (GPER) mediates non-genomic estrogen effects.
- Three GPER variants (GPER-v2, GPER-v3, GPER-v4) are known, but their individual expressions are often studied collectively.
- Understanding GPER variant expression is crucial for elucidating its physiological and pathological significance.
Purpose of the Study:
- To identify and characterize a novel GPER mRNA variant (uc010ksd.1).
- To investigate the expression of this novel variant in human cancer cell lines.
Main Methods:
- Bioinformatic analysis of the UCSC Genome Browser for novel transcripts.
- Reverse Transcription Polymerase Chain Reaction (RT-PCR) using primers specific to the novel transcript (uc010ksd.1).
Main Results:
- A novel GPER mRNA variant, designated GPER-v5, was identified with a unique 5'UTR.
- GPER-v5 expression was confirmed in human cancer cell lines: MCF-7, SW-620, COLO-205, and HT-29.
Conclusions:
- GPER-v5 represents a novel GPER mRNA variant.
- Expression of GPER-v5 in primary cells and tissues warrants further investigation to understand its potential roles.
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