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Related Experiment Video

Updated: Nov 15, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

CircRNA May Not Be "Circular".

Handong Sun¹, Zijuan Wu^2,3,4, Ming Liu⁵

¹Department of Breast Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Frontiers in Genetics

|March 8, 2021

Summary

This summary is machine-generated.

Circular RNAs (circRNAs) possess internal base-pairing sequences, suggesting complex structures beyond simple circles. This finding supports a new "open-close effect" hypothesis for circRNA molecular mechanisms.

Keywords:

bio-informatics biological phenomena circular RNA hypothesis and theory molecular conformation

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

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Last Updated: Nov 15, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Circular RNAs (circRNAs) are novel regulatory non-coding RNAs involved in various biological processes.
The precise structures and molecular mechanisms governing circRNA function remain largely undefined.

Purpose of the Study:

To investigate the structural characteristics of circRNAs using bioinformatics analysis.
To propose a novel hypothesis regarding the dynamic molecular mechanisms of circRNAs.

Main Methods:

Analysis of publicly available circRNA sequence data.
Bioinformatic identification of internal complementary base-pairing sequences (ICBPS).

Main Results:

Numerous ICBPS were identified within various circRNAs, particularly in extremely long circRNAs (el-circRNAs).
The presence of ICBPS suggests that circRNAs may exhibit more complex structures than previously assumed.
A hypothesis termed the "open-close effect" was proposed to explain potential state transitions in specific circRNAs.

Conclusions:

The study expands the understanding of circRNA structural diversity.
The findings highlight potential novel molecular mechanisms underlying circRNA function in health and disease.