Related Experiment Video
Updated: Nov 15, 2025
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Antibiotic Resistance Pattern in Pseudomonas aeruginosa Isolated from Clinical Samples Other than Burn Samples in
Ebrahim Karimi1, Fatemeh Ghalibafan2, Akram Esfandani3
1Emergency Department, Be'sat Hospital, AJA University of Medical Sciences, Tehran, Iran.
Background:
The purpose of this study was to systematically review the prevalence of class 1 integrons, antibiotic resistance pattern in Pseudomonas aeruginosa (P. aeruginosa) isolated from clinical samples other than burn samples.
Methods:
The Web of Science, PubMed, Scopus, and Science Direct databases were searched using keywords based on the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. The cross-sectional studies published from 1st January 2000 until 1st January 2019 were included which addressed the prevalence of class 1 integrons and antibiotic-resistance in P. aeruginosa isolated from clinical samples other than burn samples. Meta-analysis was conducted using Comprehensive Meta-Analysis (CMA) software. The random-effects model, Cochran's Q and I2 tests were applied for statistical analyses.
Results:
Eight articles met the eligibility standards for including in the present meta-analysis. The combined prevalence of class 1 integrons in P. aeruginosa isolated from clinical samples other than burn samples was reported by 40% (95% CI:26.1-55.8%). The pooled prevalence of Multi-Drug Resistant (MDR) P. aeruginosa isolates was 70.1%. The highest prevalence of combined antibiotic resistance was related to carbenicillin with a resistance rate of 79.9%. In general, 6 (75%) out of the 8 included studies showed the correlation between the presence of class 1 integrons and antibiotic resistance.
Conclusion:
Regarding the correlation between the presence of integrons and the high antibiotic resistance reported by studies included in the present review, there is the need for preventive measures to prevent the spread of resistance by integrons and transferring to other micro-organisms.
Insights
Class 1 integrons are prevalent in Pseudomonas aeruginosa, with a 40% occurrence in clinical samples. This indicates a need for preventive measures against antibiotic resistance spread.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Genetics
Background:
- Class 1 integrons are genetic elements conferring antibiotic resistance.
- Pseudomonas aeruginosa is an opportunistic pathogen frequently associated with hospital-acquired infections.
- Understanding the prevalence of class 1 integrons in P. aeruginosa is crucial for infection control.
Purpose of the Study:
- To systematically review the prevalence of class 1 integrons in Pseudomonas aeruginosa (P. aeruginosa) from clinical samples (excluding burn samples).
- To analyze the antibiotic resistance patterns associated with P. aeruginosa isolates carrying class 1 integrons.
- To assess the correlation between class 1 integrons and multidrug resistance in P. aeruginosa.
Main Methods:
- Systematic literature search of Web of Science, PubMed, Scopus, and Science Direct databases (2000-2019).
- Inclusion of cross-sectional studies on P. aeruginosa from clinical samples (non-burn).
- Meta-analysis using Comprehensive Meta-Analysis (CMA) software with random-effects model.
Main Results:
- Eight studies met eligibility criteria.
- Combined prevalence of class 1 integrons in P. aeruginosa was 40%.
- Pooled prevalence of multidrug-resistant (MDR) P. aeruginosa was 70.1%, with highest resistance to carbenicillin (79.9%).
- 75% of studies indicated a correlation between class 1 integrons and antibiotic resistance.
Conclusions:
- A significant prevalence of class 1 integrons and MDR P. aeruginosa was observed.
- The presence of integrons is linked to high antibiotic resistance rates.
- Preventive strategies are necessary to curb the spread of integron-mediated resistance.
