Synchronous bursting of locus coeruleus neurons in tissue culture

Synchronous bursts of firing of locus coeruleus neurons have been observed in unanesthetized rats, particularly in response to various sensory stimuli. In explant tissue cultures, synchronous bursting activity of locus coeruleus neurons was also observed and the possible mechanisms responsible for this synchronous activation have been investigated. Barrages of depolarizing events apparently initiated and continued throughout spontaneous bursts of spikes in the cultured neurons. Simultaneous intracellular recordings from pairs of neurons show a very high degree of synchrony of such barrages between cells. On the basis of tests for electrical coupling in simultaneously recorded cell pairs, and tests for dye coupling with Lucifer Yellow, it was concluded that the synchrony is not due to electrical coupling of locus coeruleus neurons. Small non-synaptic interactions between cell pairs that may reflect elevated extracellular potassium levels have been observed on some occasions. Spontaneous and evoked depolarizations similar to those initiating the bursts appear to be synaptically mediated events, suggesting that locus coeruleus neurons are synchronously activated by a common excitatory input. It was concluded that the neurons providing this common excitation are located within or very close to the locus coeruleus, at least at birth. The synchronization of activation of many locus coeruleus neurons could result in almost simultaneous release of neurotransmitter in the widespread target areas of locus coeruleus projections.