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Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Fluorescent bacterial biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz radiation
Danil S Serdyukov1,2,3, Tatiana N Goryachkovskaya1,2, Irina A Mescheryakova1,2
1Laboratory of Molecular Biotechnologies of Federal research center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 10 Lavrentiev Aven., Novosibirsk, 630090, Russia.
Abstract:
A fluorescent biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli (E. coli) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli.
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