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Updated: Nov 14, 2025

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Laser-Induced Shockwave (LIS) to Study Neuronal Ca2+ Responses.

Veronica Gomez Godinez1, Vikash Morar1, Christopher Carmona1

  • 1Institute of Engineering in Medicine, University of California, San Diego, San Diego, CA, United States.

Frontiers in Bioengineering and Biotechnology
|March 8, 2021
PubMed
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Laser-induced shockwaves (LIS) model traumatic brain injury, revealing immediate calcium ion (Ca2+) responses in neurons and Schwann cells using biosensors. This method monitors cellular stress and damage following injury.

Area of Science:

  • Neuroscience
  • Biophysics
  • Cell Biology

Background:

  • Blast-induced traumatic brain injury (TBI) involves complex cellular responses.
  • Laser-induced shockwaves (LIS) offer a controlled method to simulate TBI conditions.
  • Genetically encoded biosensors enable real-time monitoring of molecular events during injury.

Purpose of the Study:

  • To investigate the immediate calcium ion (Ca2+) response in cortical neurons and Schwann cells subjected to LIS.
  • To assess the utility of the D3CPV Ca2+ FRET biosensor in studying cellular responses to mechanical stress.
  • To correlate LIS-induced cellular responses with simulated TBI conditions.

Main Methods:

  • Utilized laser-induced shockwaves (LIS) to apply mechanical stress to cells.
Keywords:
blast induced traumacavitation bubblelaser induced shockwaveneuronal calciumshockwavetraumatic brain injury

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  • Employed the genetically encoded Ca2+ FRET biosensor D3CPV for real-time Ca2+ monitoring.
  • Examined Ca2+ transients in cortical neurons and Schwann cells under varying extracellular Ca2+ conditions.

    • Main Results:

    • Both cortical neurons and Schwann cells exhibited a transient increase in intracellular Ca2+ upon LIS exposure.
    • This Ca2+ response occurred independently of extracellular Ca2+ availability.
    • LIS facilitated simultaneous observation of shear stress effects, cell damage, and cell death.

    Conclusions:

    • LIS is a viable tool for studying the immediate cellular Ca2+ dynamics following TBI-like mechanical insults.
    • The D3CPV biosensor effectively captures rapid Ca2+ signaling events in response to LIS.
    • LIS enables comprehensive monitoring of cellular responses, including stress, damage, and death, in a controlled experimental setting.