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Updated: Nov 14, 2025
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
Antimicrobial-resistant enterobacteria in surface waters with fecal contamination from urban and rural communities
Vanessa Tibolla Moretto1, Soraia Machado Cordeiro2, Patricia Salcedo Bartley3
1Fundação Oswaldo Cruz, Instituto Gonçalo Moniz, Laboratório de Patologia e Biologia Celular, Salvador, BA, Brasil.
Introduction:
Inadequate wastewater treatment and fecal contamination have a strong environmental impact on antimicrobial resistance (AMR). This study evaluated the profile of AMR enterobacteria and fecal contamination from four surface waters: Jiquiriça-Brejões River and Cabrito, Tororó, and Abaeté Lagoons.
Methods:
We analyzed AMR β-lactamase genes using the polymerase chain reaction method and fecal contamination using Coliscan®.
Results:
We found high levels of fecal contamination, β-lactamase producers, and AMR genes (blaOXA-48, blaSPM, and blaVIM) in all waterbodies.
Conclusions:
Poor sanitation evidenced by fecal contamination and human activities around these surface waters contributed to the distribution and increase in AMR enterobacteria.
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