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Determination and validation of aprepitant in rat plasma using LC-MS/MS.

Nazlı Erdoğar1, Tuba Reçber2, Alper Bektas İskit3

  • 1Department of Pharmaceutical Technology, Hacettepe University, Faculty of Pharmacy, Sıhhiye-Ankara 06100, Turkey.

Bioanalysis
|March 8, 2021
PubMed
Summary

A validated bioanalytical method using LC-MS/MS was developed for quantifying aprepitant in rat plasma. This method ensures accurate pharmacokinetic assessments, crucial for drug efficacy studies.

Keywords:
LC−MS/MSaprepitantpharmacokineticsplasmavalidation

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Bioanalysis

Background:

  • Accurate pharmacokinetic assessments are essential for evaluating drug efficacy.
  • Validated bioanalytical methods are prerequisites for reliable plasma concentration measurements.

Purpose of the Study:

  • To develop and validate a sensitive LC-MS/MS method for the quantitative analysis of aprepitant in rat plasma.
  • To ensure the reliability of pharmacokinetic data for aprepitant.

Main Methods:

  • A simple, specific, rapid, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established.
  • A C18 column was utilized with a mobile phase comprising formic acid in water and acetonitrile.
  • Quantification was performed using multiple reaction monitoring (MRM).

Main Results:

  • The developed LC-MS/MS method demonstrated excellent selectivity, linearity, accuracy, and precision.
  • Robustness and ruggedness evaluations confirmed the method's reliability according to ICH guidelines.
  • All validation parameters were within acceptable ranges, indicating a robust analytical procedure.

Conclusions:

  • The validated LC-MS/MS method is suitable for the accurate quantitative analysis of aprepitant in rat plasma.
  • This method supports reliable pharmacokinetic studies and drug efficacy assessments.