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Determination and validation of aprepitant in rat plasma using LC-MS/MS
Nazlı Erdoğar1, Tuba Reçber2, Alper Bektas İskit3
1Department of Pharmaceutical Technology, Hacettepe University, Faculty of Pharmacy, Sıhhiye-Ankara 06100, Turkey.
Bioanalysis
|March 8, 2021
Summary
A validated bioanalytical method using LC-MS/MS was developed for quantifying aprepitant in rat plasma. This method ensures accurate pharmacokinetic assessments, crucial for drug efficacy studies.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Bioanalysis
Background:
- Accurate pharmacokinetic assessments are essential for evaluating drug efficacy.
- Validated bioanalytical methods are prerequisites for reliable plasma concentration measurements.
Purpose of the Study:
- To develop and validate a sensitive LC-MS/MS method for the quantitative analysis of aprepitant in rat plasma.
- To ensure the reliability of pharmacokinetic data for aprepitant.
Main Methods:
- A simple, specific, rapid, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established.
- A C18 column was utilized with a mobile phase comprising formic acid in water and acetonitrile.
- Quantification was performed using multiple reaction monitoring (MRM).
Main Results:
- The developed LC-MS/MS method demonstrated excellent selectivity, linearity, accuracy, and precision.
- Robustness and ruggedness evaluations confirmed the method's reliability according to ICH guidelines.
- All validation parameters were within acceptable ranges, indicating a robust analytical procedure.
Conclusions:
- The validated LC-MS/MS method is suitable for the accurate quantitative analysis of aprepitant in rat plasma.
- This method supports reliable pharmacokinetic studies and drug efficacy assessments.
