Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Nov 14, 2025

Creating Avian Forebrain Chimeras to Assess Facial Development

Creating Avian Forebrain Chimeras to Assess Facial Development

Published on: February 18, 2021

Creating Avian Forebrain Chimeras to assess Facial Development.

Diane Hu¹, Ralph S Marcucio²

¹Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, University of California.

Journal of Visualized Experiments : Jove

|March 8, 2021

Summary

Avian embryos reveal how forebrain signals guide facial development by shaping Sonic hedgehog (SHH) expression. This study details methods for creating chimeric embryos to study these crucial developmental interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Three-dimensional cellular dynamics and mandibular morphogenesis.

Frontiers in cell and developmental biology·2026

Same author

Murine Progeria Model Exhibits Delayed Fracture Healing With Senescent Phenotype and Dysregulated Immune Response.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026

Same author

Age-related Delays in Osteochondral Remodeling of Fracture Healing Illustrated by Mass Spectrometry Imaging.

bioRxiv : the preprint server for biology·2026

Same author

Enhanced fracture repair in p21<sup>-/-</sup> mice is mediated through increased callus mineralization.

Bone·2025

Same author

What's New in Musculoskeletal Basic Science.

The Journal of bone and joint surgery. American volume·2025

Same author

Mesenchymal progenitor-derived proteoglycan 4 regulates the transdifferentiation of chondrocytes into osteoblasts during fracture healing.

Stem cells translational medicine·2025

Area of Science:

Developmental biology
Comparative embryology
Molecular signaling in vertebrates

Background:

The avian embryo is a long-standing model for vertebrate development.
Chimeric embryos allow direct assessment of tissue interactions.
Previous work identified forebrain signals influencing facial morphogenesis via Sonic hedgehog (SHH).

Purpose of the Study:

To describe the methodology for generating forebrain chimeric avian embryos.
To illustrate the experimental outcomes of these chimeric embryo studies.
To further elucidate the role of forebrain-derived signals in facial development.

Main Methods:

Generation of avian chimeric embryos by combining tissues from different genetic sources.
Analysis of facial morphogenesis in chimeric embryos.

More Related Videos

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras

Published on: May 31, 2014

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Published on: March 24, 2011

Related Experiment Videos

Last Updated: Nov 14, 2025

Creating Avian Forebrain Chimeras to Assess Facial Development

Creating Avian Forebrain Chimeras to Assess Facial Development

Published on: February 18, 2021

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras

Published on: May 31, 2014

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Published on: March 24, 2011

Assessment of gene expression patterns, specifically Sonic hedgehog (SHH), in the Frontonasal Ectodermal Zone (FEZ).

Main Results:

Successful generation of forebrain chimeric embryos.
Demonstration of forebrain's influence on the shape of the SHH expression domain in the FEZ.
Visual evidence of developmental interactions between forebrain and facial structures.

Conclusions:

Forebrain signals are critical regulators of facial morphogenesis in avian development.
The described chimera generation method is a valuable tool for studying developmental interactions.
Understanding SHH regulation by forebrain signals provides insight into vertebrate craniofacial development.