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Inflammation

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Related Experiment Video

Updated: Nov 14, 2025

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

Published on: May 17, 2021

Modeling Skin Inflammation Using Human In Vitro Models.

Erika Boarder¹, Beth Rumberger¹, Michael D Howell²

¹Incyte Research Institute, Wilmington, Delaware.

Current Protocols

|March 8, 2021

Summary

This summary is machine-generated.

This study introduces new human skin models using keratinocytes to investigate inflammatory skin diseases like psoriasis. These models help researchers understand how tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) cause inflammation.

Keywords:

genomics inflammation keratinocytes proteomics skin

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Last Updated: Nov 14, 2025

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

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Published on: May 17, 2021

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Published on: March 28, 2025

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Area of Science:

Dermatology and cell biology
Investigating skin barrier function and inflammation

Background:

Skin's epidermis and dermis form a protective barrier.
Keratinocytes constitute over 95% of the epidermis.
Inflammatory skin diseases involve barrier disruption and inflammation.

Purpose of the Study:

To present protocols for human skin models to study inflammatory skin diseases.
To investigate the effects of TNF-α and IFN-γ on skin cells.
To provide models for conditions like psoriasis and vitiligo.

Main Methods:

Utilizing HaCaT keratinocyte cultures.
Developing 3-dimensional organotypic skin cultures.
Assessing TNF-α and IFN-γ driven skin inflammation.

Main Results:

Established protocols for keratinocyte culture and 3D skin models.
Demonstrated a method to study cytokine-driven inflammation in human skin.
Provided a surrogate for studying autoimmune skin diseases.

Conclusions:

Human skin models offer a viable alternative to animal models for studying inflammatory skin diseases.
These protocols facilitate research into TNF-α and IFN-γ mediated skin inflammation.
The developed models can advance understanding of psoriasis, vitiligo, and related conditions.