Related Experiment Video
Updated: Nov 14, 2025
09:30
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
9.2K
Maturation system affects lipid accumulation in bovine oocytes.
O A C Faria1, T S Kawamoto2, L R O Dias1
1Departament of Animal Science, University of Brasilia, Animal Science, Brasília, Distrito Federal, 70910-900, Brazil.
Reproduction, Fertility, and Development
|March 8, 2021
Summary
Oocytes matured using intrafollicular transfer (IFIOT) show lipid accumulation similar to in vivo maturation, suggesting improved quality over in vitro maturation. This finding impacts bovine oocyte maturation techniques.
Area of Science:
- Reproductive Biology
- Cell Biology
- Oocyte Maturation
Background:
- Lipid accumulation in oocytes is crucial for developmental competence.
- Current in vitro maturation (MatV) systems may alter oocyte lipid profiles.
- Alternative maturation methods require evaluation for their impact on oocyte lipid content.
Purpose of the Study:
- To compare lipid droplet accumulation in bovine oocytes matured via in vitro (MatV), in vivo (MatS), and intrafollicular transfer (IFIOT; MatT) systems.
- To investigate the expression of key lipid metabolism genes (ACSS2, ELOVL1, FABP3) across different maturation systems.
- To assess the impact of maturation systems on oocyte quality through lipid analysis.
Main Methods:
- Confocal and transmission electron microscopy for lipid droplet evaluation.
- Quantitative polymerase chain reaction (qPCR) for gene expression analysis of ACSS2, ELOVL1, and FABP3.
- Comparison of lipid content and gene expression across MatV, MatS, and IFIOT groups.
Main Results:
- Oocytes matured in vitro (MatV) exhibited significantly higher lipid accumulation (24±2%) compared to in vivo (MatS, 16±2%) and IFIOT (MatT, 12±2%) groups.
- Ultrastructural analysis confirmed higher lipid content in MatV oocytes.
- Expression of ELOVL1 and FABP3 was similar between MatS and IFIOT, while ACSS2 was lower in IFIOT than MatV oocytes.
Conclusions:
- Intrafollicular transfer of immature oocytes (IFIOT) results in lipid accumulation comparable to in vivo matured oocytes.
- IFIOT represents a promising maturation system, yielding oocytes with lipid profiles superior to standard in vitro maturation (MatV).
- These findings suggest IFIOT may enhance bovine oocyte quality for assisted reproductive technologies.
Related Concept Videos
02:07Oogenesis
67.5K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
67.5K
01:22Oogenesis
3.0K
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
3.0K
01:20Folliculogenesis
1.5K
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
1.5K