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Related Concept Videos

CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Experimental RNAi02:15

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Interrogating lncRNA functions via CRISPR/Cas systems.

Meira S Zibitt1, Corrine Corrina R Hartford1, Ashish Lal1

  • 1Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA.

RNA Biology
|March 9, 2021
PubMed
Summary
This summary is machine-generated.

The CRISPR/Cas system offers innovative tools for studying long noncoding RNAs (lncRNAs), enabling researchers to explore their functions and therapeutic potential in various biological processes and diseases.

Keywords:
Lncrnacrispr/cas9genome editinglincRNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in biological processes and diseases.
  • Studying lncRNA function is challenging due to low abundance and poor conservation.

Purpose of the Study:

  • To review the application of CRISPR/Cas technology for investigating long noncoding RNA (lncRNA) function.
  • To highlight the potential of CRISPR/Cas in understanding lncRNA roles and therapeutic implications.

Main Methods:

  • Utilizing the CRISPR/Cas system for targeted DNA and/or RNA manipulation.
  • Employing CRISPR/Cas for lncRNA knockout, knockdown, overexpression, and imaging applications.

Main Results:

  • CRISPR/Cas technology provides versatile tools for lncRNA functional studies.
  • The system facilitates detailed investigation into the biological roles of lncRNAs.

Conclusions:

  • CRISPR/Cas system is a powerful technology for advancing lncRNA research.
  • This technology opens new avenues for exploring therapeutic strategies involving lncRNAs.